Key result
Human embryonic stem cell-derived cardiomyocytes show immature E-C coupling driven primarily by extracellular calcium.
Why the study?
The functional adaptability and excitation-contraction coupling properties of human embryonic stem cell-derived cardiomyocytes at 45 to 60 days developmental stage compared to adult myocardium were not established.
Population
Human embryonic stem cell-derived cardiomyocytes at 45 to 60 days developmental stage
Comparison
hESC-CM versus adult myocardium properties
Design
Preclinical functional study
Authors
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Warrants caution against hESC-CM use in cardiac regeneration; leaves open targeted maturation strategies before clinical translation.
Human embryonic stem cell-derived cardiomyocytes exhibit immature excitation-contraction coupling that relies on extracellular calcium rather than sarcoplasmic reticulum stores, suggesting a need for genetic manipulation before use in cardiac regeneration.
Dolnikov et al. (2005) studied this question. Human embryonic stem cell-derived cardiomyocytes (hESC-CM) vs. Mature/adult myocardium was evaluated on Excitation-contraction (E-C) coupling and responsiveness to physiological stimuli. Human embryonic stem cell-derived cardiomyocytes display immature excitation-contraction coupling that is dependent on extracellular calcium rather than sarcoplasmic reticulum stores.
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