What question did this study set out to answer?

To determine whether locally produced angiotensin II contributes to increased growth factor expression in vascular smooth muscle cells from spontaneously hypertensive rats.

January 1, 2001Journal of Cardiovascular Pharmacology

Role of Endogenous Angiotensin II in the Increased Expression of Growth Factors in Vascular Smooth Muscle Cells from Spontaneously Hypertensive Rats

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Why the study?

Does endogenous Angiotensin II mediate the increased expression of growth factors in vascular smooth muscle cells from spontaneously hypertensive rats?

Population

Cultured vascular smooth muscle cells derived from spontaneously hypertensive rats and normotensive…

Comparison

Treatment with isoproterenol, delapril, or CV… vs Untreated VSMC or VSMC from WKY rats

Design

Preclinical

Authors

CSChikara SatohNihon University NFNoboru FukudaUniversity of Pittsburgh WHWen‐Yang HuEast China University of Technology

Discussion

Member takes

Implication

Hypothesis-generating in SHR vascular models; leaves open translation to human hypertension therapy.

Key Points

To determine whether locally produced angiotensin II contributes to increased growth factor expression in vascular smooth muscle cells from spontaneously hypertensive rats.
Cultured vascular smooth muscle cells isolated from spontaneously hypertensive rats and normotensive Wistar-Kyoto control rats.
Measured TGF-beta1, PDGF A-chain, and bFGF mRNA levels using RT-PCR, and quantified angiotensin II-like immunoreactivity in conditioned medium via radioimmunoassay.
Evaluated the effects of isoproterenol, the ACE inhibitor delapril, and the AT1 receptor antagonist CV 11974 on growth factor expression.
Basal levels of angiotensin II-like immunoreactivity and mRNAs for TGF-beta1, PDGF A-chain, and bFGF were significantly higher in cells from spontaneously hypertensive rats than Wistar-Kyoto controls.
Isoproterenol significantly increased angiotensin II immunoreactivity in both strains and elevated angiotensinogen, TGF-beta1, PDGF A-chain, and bFGF mRNA levels in hypertensive cells.
Treatment with delapril or CV 11974 markedly suppressed the elevated mRNA and protein levels of TGF-beta1, PDGF, and bFGF in cells from spontaneously hypertensive rats.

Structured PICO

Does endogenous Angiotensin II mediate the increased expression of growth factors in vascular smooth muscle cells from spontaneously hypertensive rats?

Population

Cultured vascular smooth muscle cells (VSMC) derived from spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats

Intervention

Treatment with isoproterenol, delapril (ACE inhibitor), or CV 11974 (Ang II type 1 receptor antagonist)

Comparator

Untreated VSMC or VSMC from WKY rats

Outcome

Expression levels of TGF-beta1, PDGF A-chain, and bFGF mRNAs and proteins, and Ang II-like immunoreactivitysurrogate

Endogenous Angiotensin II contributes to the exaggerated growth of vascular smooth muscle cells in spontaneously hypertensive rats by enhancing the expression of growth factors like TGF-beta1, PDGF A-chain, and bFGF.

Cite This Study

Satoh et al. (2001) studied this question.

synapsesocial.com/papers/6a786495c5ab23c76ced4d88 https://doi.org/10.1097/00005344-200101000-00013

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Role of Endogenous Angiotensin II in the Increased Expression of Growth Factors in Vascular Smooth Muscle Cells from Spontaneously Hypertensive Rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Role of Endogenous Angiotensin II in the Increased Expression of Growth Factors in Vascular Smooth Muscle Cells from Spontaneously Hypertensive Rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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