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August 1, 1999Journal of Hypertension

Contribution of synthetic phenotype on the enhanced angiotensin II-generating system in vascular smooth muscle cells from spontaneously hypertensive rats

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Key result

Cultured vascular smooth muscle cells from spontaneously hypertensive rats exhibit a synthetic phenotype early in culture with increased expression of Ang II-generating components and growth factors.

Population

Cultured vascular smooth muscle cells from spontaneously hypertensive rats and normotensive Wistar-Kyoto rats

Comparison

Cell passage to observe changes in cell phenotype vs VSMC from normotensive Wistar-Kyoto (WKY) rats

Design

Preclinical

Authors

NFNoboru FukudaUniversity of PittsburghWHWen-Yang HuUniversity of Illinois ChicagoCSChikara SatohNihon University

Discussion

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Overview

Local Ang II production in SHR VSMC may promote growth; leaves open in vivo and human relevance.

Structured PICO

P
Population
In vitro study evaluating cultured vascular smooth muscle cells from spontaneously hypertensive rats compared to normotensive Wistar-Kyoto rats across multiple passages.
E
Exposure
Cell passage (passages 4, 8, and 12) to observe changes in cell phenotype
C
Comparator
VSMC from normotensive Wistar-Kyoto (WKY) rats
O
Outcome
Basal DNA synthesis, immunofluorescence of alpha-smooth muscle actin, mRNA expression of phenotype markers, Ang II-generating system components, growth factors, and Ang II levelssurrogate

Vascular smooth muscle cells from spontaneously hypertensive rats inherently display an early synthetic phenotype with enhanced Angiotensin II generation, driving exaggerated cell growth.

Cite This Study

Fukuda et al. (1999) studied Hypertension. Spontaneously hypertensive rat (SHR) strain vs. Normotensive Wistar-Kyoto (WKY) rat strain was evaluated on Basal DNA synthesis, phenotype markers, Ang II-generating system components, and growth factors. Cultured vascular smooth muscle cells from spontaneously hypertensive rats exhibit a synthetic phenotype early in culture with increased expression of Ang II-generating components and growth factors.

synapsesocial.com/papers/6a6cb5ab53da1ffdb5078786https://doi.org/10.1097/00004872-199917080-00009
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inhibitory Effect of an Angiotensin II Type 1 Receptor Antagonist on Growth of Vascular Smooth Muscle Cells from Spontaneously Hypertensive Rats1996 · 38 citations
  2. 2Developmental changes in expression of contractile and cytoskeletal proteins in human aortic smooth muscle.1990 · 138 citations
  3. 3Transforming growth factor beta 1 expression and effect in aortic smooth muscle cells from spontaneously hypertensive rats.1991 · 71 citations
  4. 4Molecular cloning of matrix Gla protein: implications for substrate recognition by the vitamin K-dependent gamma-carboxylase.1987 · 165 citations
  5. 5Transforming growth factor-beta and p27 expression in pituitary cells.1996 · 101 citations