What question did this study set out to answer?

To determine the inhibitory effects of [Sar(1)-Ile(8)]-angiotensin II on aldosterone biosynthesis stimulated by angiotensin II, des-Asp(1)-angiotensin II, and adrenocorticotropic hormone.

February 1, 1974Proceedings of the National Academy of SciencesOpen Access

Inhibition of Induced Aldosterone Biosynthesis with a Specific Antagonist of Angiotensin II

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Population

Rabbit adrenal cortical cell suspensions from male albino rabbits weighing 2.5-3.5 kg.

Comparison

[Sar-Ile]-angiotensin II added 2 min before… vs Steroidogenic peptides alone (angiotensin II…

Design

Preclinical

Follow-up

90 minutes

Authors

ACAndrew T. ChiuHong Kong Polytechnic University MPM J PeachLancaster University

Discussion

Member takes

Implication

Selectively blocks angiotensin- but not ACTH-stimulated aldosterone in rabbit cells; leaves open human translation and therapeutic relevance.

Key Points

To determine the inhibitory effects of [Sar(1)-Ile(8)]-angiotensin II on aldosterone biosynthesis stimulated by angiotensin II, des-Asp(1)-angiotensin II, and adrenocorticotropic hormone.
Isolated rabbit adrenal capsular cortical cells using collagenase digestion.
Assessed aldosterone production via immunoassay following exposure to steroidogenic peptides in the presence or absence of [Sar(1)-Ile(8)]-angiotensin II (10⁻⁷ to 5 × 10⁻⁷ M).
[Sar(1)-Ile(8)]-angiotensin II completely blocked aldosterone synthesis stimulated by angiotensin II and des-Asp(1)-angiotensin II without altering basal biosynthesis or adrenocorticotropic hormone-induced synthesis.
Achieving 100% inhibition required an antagonist-to-agonist dose ratio of approximately 2:1 against angiotensin II and approximately 50:1 against des-Asp(1)-angiotensin II.

Structured PICO

Population

Rabbit adrenal cortical cell suspensions (zona glomerulosa cells) from male albino rabbits weighing 2.5-3.5 kg.

Intervention

[Sar(1)-Ile(8)]-angiotensin II (10^-7 to 5 x 10^-7 M) added 2 min before steroidogenic peptides (angiotensin II, des-Asp(1)-angiotensin II, or ACTH)

Comparator

Steroidogenic peptides alone (angiotensin II, des-Asp(1)-angiotensin II, or ACTH) and drug solvent control

Outcome

Aldosterone biosynthesis/levels determined by immunoassay at 90 minutes of incubationsurrogate

[Sar(1)-Ile(8)]-angiotensin II specifically inhibits aldosterone biosynthesis induced by angiotensin II and its heptapeptide metabolite, indicating the heptapeptide has a higher affinity for the adrenal cortex receptor.

Cite This Study

Chiu et al. (1974) studied this question.

synapsesocial.com/papers/6a789e4c576efb0ddaf83589 https://doi.org/10.1073/pnas.71.2.341

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Implication

Selectively blocks angiotensin- but not ACTH-stimulated aldosterone in rabbit cells; leaves open human translation and therapeutic relevance.

Inhibition of Induced Aldosterone Biosynthesis with a Specific Antagonist of Angiotensin II

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Inhibition of Induced Aldosterone Biosynthesis with a Specific Antagonist of Angiotensin II

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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