April 1, 1999Journal of Biological ChemistryOpen Access

Cyclic GMP-dependent Protein Kinase Activates Cloned BKCa Channels Expressed in Mammalian Cells by Direct Phosphorylation at Serine 1072

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Population

HEK293 cells expressing the cDNA encoding the alpha-subunit of the large conductance Ca2+-activated K+ channel

Comparison

NO-donor sodium nitroprusside and direct… vs Control conditions, PKG-inhibitor KT5823, and…

Design

Preclinical

Authors

MFMitsuhiro FukaoMusashino University HMHelen S. MasonBirmingham Community Healthcare NHS Trust FBFiona C. BrittonUniversity of Nevada, Las Vegas

Discussion

Member takes

Implication

Confirms direct PKG phosphorylation of BK channels at Ser-1072 in vitro; leaves open relevance to human vascular physiology.

Structured PICO

Population

HEK293 cells expressing the cDNA encoding the alpha-subunit of the large conductance Ca2+-activated K+ channel (cslo-alpha)

Intervention

NO-donor sodium nitroprusside (SNP) and direct application of PKG-Ialpha

Comparator

Control conditions, PKG-inhibitor KT5823, and mutant cslo-alpha (Ser-1072 replaced by Ala)

Outcome

Channel open probability (NPo) and outward currentsurrogate

PKG activates cloned large conductance Ca2+-activated K+ channels by direct phosphorylation at serine 1072, elucidating the molecular mechanism of NO-induced smooth muscle relaxation.

Cite This Study

Fukao et al. (1999) studied this question.

synapsesocial.com/papers/6a7a2cc58a3ae1c74232a211 https://doi.org/10.1074/jbc.274.16.10927

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Cyclic GMP-dependent Protein Kinase Activates Cloned BKCa Channels Expressed in Mammalian Cells by Direct Phosphorylation at Serine 1072

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cyclic GMP-dependent Protein Kinase Activates Cloned BKCa Channels Expressed in Mammalian Cells by Direct Phosphorylation at Serine 1072

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider