Overexpression of a human potassium channel suppresses cardiac hyperexcitability in rabbit ventricular myocytes
View Full Paper
Ask AI
Bookmark
Share
Why the study?
Does adenoviral gene transfer of HERG suppress early afterdepolarizations and enhance repolarization in cultured adult rabbit ventricular myocytes?
Population
Adult rabbit ventricular myocytes maintained in primary culture, and Chinese hamster ovary cells.
Comparison
Adenoviral gene transfer of the human K+ channel… vs Noninfected myocytes or myocytes infected with a…
Design
Preclinical
Follow-up
36-72 hours post-isolation (in vitro)
Authors
Discussion
Loading...
Member takes
Implication
HERG gene transfer may suppress EADs in myocytes; hypothesis-generating in this animal model and should not yet change practice.
Structured PICO
Does adenoviral gene transfer of HERG suppress early afterdepolarizations and enhance repolarization in cultured adult rabbit ventricular myocytes?
P
Population
Adult rabbit ventricular myocytes maintained in primary culture (which prolongs action potentials and predisposes to early afterdepolarizations), and Chinese hamster ovary (CHO-K1) cells.
I
Intervention
Adenoviral gene transfer of the human K+ channel HERG (AdHERG) driven by a Rous sarcoma virus (RSV) promoter.
C
Comparator
Noninfected myocytes or myocytes infected with a control reporter adenovirus (AdGFP).
O
Outcome
Action potential duration (APD90), occurrence of early afterdepolarizations (EADs), and relative refractory period (RRP).surrogate
Overexpression of the HERG potassium channel via gene transfer successfully shortens action potential duration, suppresses arrhythmogenic early afterdepolarizations, and prolongs the refractory period in ventricular myocytes.
Cite This Study
Nuss et al. (1999) studied this question.
View Full Paper
Ask AI
Bookmark
Share