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Purify and characterize the physical, chemical, and kinetic properties of renin substrates derived from sheep and human plasma.

February 1, 1975Immunology and Cell Biology

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

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Authors

SSSL SkinnerThe University of Melbourne JDJR DunnThe University of Melbourne JMJ MazzettiThe University of Melbourne

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Implication

Comparative biochemical study demonstrates kinetic differences between sheep and human renin substrates, indicating substrate levels limit in vivo angiotensin production.

Key Points

Purify and characterize the physical, chemical, and kinetic properties of renin substrates derived from sheep and human plasma.
Purified sheep plasma renin substrate using nephrectomized sheep plasma, DEAE-Sephadex chromatography, and gel filtration.
Purified human renin substrate from plasma of pregnant women at 36–40 weeks' gestation using DEAE-Sephadex chromatography.
Assessed molecular weights, susceptibility to peptic and renin digestion, and Michaelis constants (Km) across homologous and heterologous enzyme-substrate reactions at pH 7.5.
Sheep renin substrate was purified 1,200-fold (MW ~52,000; 8 µg angiotensin II/mg protein), displaying a Km of 0.29 µM with human renin and 2.0 µM with sheep renin.
Human renin substrate was purified 70-fold (MW ~82,000; 0.9 µg angiotensin II/mg protein), was resistant to sheep renin and pepsin, and exhibited a mean Km of 5.7 µM (range 3–8 µM) with human renin.
Normal physiological concentrations of renin substrate in both sheep and humans remain well below the measured Km values, establishing substrate concentration as a key rate determinant of angiotensin production in vivo.

Cite This Study

Skinner et al. (1975) studied this question.

synapsesocial.com/papers/6a7cd87ce9867978b6a1e270 https://doi.org/10.1038/icb.1975.8

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Implication

Comparative biochemical study demonstrates kinetic differences between sheep and human renin substrates, indicating substrate levels limit in vivo angiotensin production.

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

Discussion

Member takes

Implication

Key Points

Cite This Study

Purification, Properties and Kinetics of Sheep and Human Renin Substrates

Discussion

Member takes

Implication

Key Points

Cite This Study

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