September 1, 1996AJP Heart and Circulatory Physiology
Phorbol 12-myristate 13-acetate alters SR Ca(2+)-ATPase gene expression in cultured neonatal rat heart cells
Why the study?
Does phorbol 12-myristate 13-acetate alter SERCA2 gene expression and Ca2+ transient decline in cultured neonatal rat ventricular myocytes?
Population
Primary cultures of neonatal rat ventricular myocytes
Comparison
Phorbol 12-myristate 13-acetate vs Control myocytes
Design
Preclinical
Follow-up
Up to 72 hours
Authors
Discussion
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Member takes
Implication
Hypothesis-generating for PKC-mediated SERCA2 loss in hypertrophy models; leaves open translation to adult human HF.
Structured PICO
Does phorbol 12-myristate 13-acetate alter SERCA2 gene expression and Ca2+ transient decline in cultured neonatal rat ventricular myocytes?
P
Population
Primary cultures of neonatal rat ventricular myocytes
I
Intervention
Phorbol 12-myristate 13-acetate (PMA) (200 nM, 30 min to 72 h)
C
Comparator
Control myocytes
O
Outcome
Cytoplasmic Ca2+ ([Ca2+]i) transient decline half-time and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2) gene expression (protein and mRNA levels)surrogate
PMA downregulates SERCA2 gene expression in cultured neonatal rat myocytes, partly by altering mRNA stability, leading to functional alterations in Ca2+ decline similar to those observed in hypertrophied and failing adult myocardium.
Cite This Study
Qi et al. (1996) studied this question.