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August 1, 1994Cardiovascular Research

Preconditioning human ventricular cardiomyocytes with brief periods of simulated ischaemia

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Why the study?

Does ischaemic preconditioning reduce cellular injury in human ventricular cardiomyocytes exposed to sustained ischaemia?

Population

Monolayer cultures of quiescent human ventricular cardiomyocytes (n = 8 plates per group)

Comparison

Preconditioning with varying periods of… vs Control ischaemia followed by 90 min of…

Design

Preclinical

Follow-up

24 h postintervention

Authors

JIJohn S. IkonomidisCardiac SurgeryLTL. C TumiatiToronto Western HospitalRWRichard D. WeiselUniversity of Toronto

Discussion

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Implication

Indicates direct cardiomyocyte protection by preconditioning; leaves open translation to human clinical ischaemia.

Structured PICO

Does ischaemic preconditioning reduce cellular injury in human ventricular cardiomyocytes exposed to sustained ischaemia?

P
Population
Monolayer cultures of quiescent human ventricular cardiomyocytes (n = 8 plates per group)
I
Intervention
Preconditioning with varying periods of simulated ischaemia and reperfusion (e.g., 20 min ischaemia and 20 min reperfusion), followed by 90 min of sustained ischaemia with or without 30 min of reperfusion
C
Comparator
Control ischaemia (no preconditioning) followed by 90 min of sustained ischaemia with or without 30 min of reperfusion
O
Outcome
Cellular injury assessed by trypan blue exclusion and survival assessed by culturing the cells for 24 h postinterventionsurrogate

Ischaemic preconditioning can be induced directly in human ventricular cardiomyocytes independent of other cell types, reducing cellular injury during subsequent sustained ischaemia.

Cite This Study

Ikonomidis et al. (1994) studied this question.

synapsesocial.com/papers/6a7d2509d685fe2c2d775848https://doi.org/10.1093/cvr/28.8.1285
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Preconditioning the human myocardium by simulated ischemia: studies on the early and delayed protection2000 · 84 citations
  2. 2Preconditioning of isolated rabbit cardiomyocytes: effects of glycolytic blockade, phorbol esters, and ischaemia1994 · 61 citations
  3. 3Differential effect of preconditioning on post-ischaemic myocardial performance in the absence of substantial infarction and in extensively infarcted rat hearts2001 · 9 citations
  4. 4Optimal Myocardial Preconditioning in Humansa1999 · 5 citations
  5. 5Ischemic preconditioning: antiarrhythmic effects and electrophysiological mechanisms in isolated ventricle1998 · 23 citations