Human immunodeficiency virus tat-activated expression of poliovirus protein 2A inhibits mRNA translation.
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Population
HeLa cells and in vitro translation systems (rabbit reticulocyte lysate)
Comparison
Expression of poliovirus protein 2A activated by… vs Unactivated cells or mutant 2A constructs
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May inform antiviral protease targeting; leaves open clinical translation from this model system.
Structured PICO
P
Population
HeLa cells and in vitro translation systems (rabbit reticulocyte lysate)
I
Intervention
Expression of poliovirus protein 2A activated by HIV tat protein
C
Comparator
Unactivated cells (without tat expression) or mutant 2A constructs
O
Outcome
Cellular mRNA translation (measured by chloramphenicol acetyltransferase synthesis) and cleavage of P220surrogate
Poliovirus protein 2A alone can inhibit cellular protein synthesis and induce cleavage of P220, and the HIV tat activation system provides an effective method to control gene expression in mammalian cells.
Cite This Study
Sun et al. (1989) studied this question.
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