June 1, 1988Molecular and Cellular BiologyOpen Access

Upstream Regulatory Region for Inducible Expression of the Chicken Skeletal Myosin Alkali Light-Chain Gene

View Full Paper

Ask AI

Bookmark

Population

Quail myoblasts transformed by a temperature-sensitive mutant of Rous sarcoma virus or chicken myoblasts

Design

Preclinical

Authors

MSMasaki ShirakataTokyo Medical and Dental University YNYo-ichi NabeshimaKyoto University KKKazuhiko KonishiHokkaido University

Discussion

Member takes

Implication

May inform myogenic gene regulation research; leaves open validation in mammalian models and clinical translation.

Structured PICO

Population

Quail myoblasts transformed by a temperature-sensitive mutant of Rous sarcoma virus (tsNY68) or chicken myoblasts

Intervention

Introduction of a fusion gene containing the 5'-flanking and upstream region of the chicken myosin alkali light-chain gene joined to the structural gene for chloramphenicol acetyltransferase (CAT), including external and internal deletion mutants

Outcome

Transiently expressed CAT activity after differentiation of myoblastssurrogate

Identified a 160-nucleotide cis-acting regulatory region located 2 kb upstream of the transcription initiation site that controls the inducible expression of the chicken skeletal myosin alkali light-chain gene during muscle cell differentiation.

Cite This Study

Shirakata et al. (1988) studied this question.

synapsesocial.com/papers/6a81670fadfa6baaaee1d2f6 https://doi.org/10.1128/mcb.8.6.2581-2588.1988

View Full Paper

Ask AI

Bookmark

Upstream Regulatory Region for Inducible Expression of the Chicken Skeletal Myosin Alkali Light-Chain Gene

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Upstream Regulatory Region for Inducible Expression of the Chicken Skeletal Myosin Alkali Light-Chain Gene

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider