July 1, 1989Circulation ResearchOpen Access

Mechanism of action of EDRF on pressurized arteries: effect on K+ conductance.

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Population

Rat femoral arteries cannulated and pressurized to 100 mm Hg, precontracted with 5 x 10(-6) M norepinephrine

Comparison

Perfusion with 5 x 10 M acetylcholine to… vs Deendothelialized vessels or absence of ACh

Design

Preclinical

Authors

Discussion

Endothelium-dependent K+ channel hyperpolarization by ACh in animals; leaves open translation to human endothelial function.

Population

Rat femoral arteries cannulated and pressurized to 100 mm Hg, precontracted with 5 x 10(-6) M norepinephrine

Intervention

Perfusion with 5 x 10(-6) M acetylcholine (ACh) to stimulate endothelium-derived relaxing factor (EDRF) release

Comparator

Deendothelialized vessels (collagenase-treated) or absence of ACh

Outcome

Vascular smooth muscle membrane potential (Em) and diameter responsessurrogate

EDRF induces vascular smooth muscle relaxation and hyperpolarization by increasing potassium conductance.

Kauser et al. (1989) studied this question.

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