What question did this study set out to answer?

To determine whether endothelium-derived relaxing factor (EDRF) modulates the release of norepinephrine from adrenergic nerve endings in isolated vascular tissue.

March 1, 1990American Journal of Hypertension

Release of Norepinephrine From Adrenergic Nerve Endings of Blood Vessels Is Modulated by Endothelium-Derived Relaxing Factor

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Population

Isolated canine pulmonary arteries and veins

Comparison

Intact endothelium vs Endothelium rubbed blood vessels

Design

Preclinical

Authors

SGStan S. GreenbergRutgers, The State University of New Jersey FDF.P.J. DieckeRutgers, The State University of New Jersey KPKeith J. PeevyUniversity of South Alabama

Discussion

Member takes

Implication

Endothelium may inhibit sympathetic NE release in isolated vessels; hypothesis-generating for EDRF in human disease.

Key Points

To determine whether endothelium-derived relaxing factor (EDRF) modulates the release of norepinephrine from adrenergic nerve endings in isolated vascular tissue.
Superfused isolated canine pulmonary artery and vein segments with intact or rubbed endothelium during transmural nerve stimulation across frequencies from 1 to 32 Hz for 10 minutes.
Measured contractile responses and the efflux of radiolabeled 2-[14C]-norepinephrine, alongside bioassays utilizing EDRF derived from porcine thoracic aorta.
Vascular segments with intact endothelium showed significantly reduced contractile responses to low-frequency transmural nerve stimulation compared to endothelium-rubbed vessels.
Electrical stimulation released less 2-[14C]-norepinephrine in intact vessels than rubbed vessels, with maximal inhibition observed at lower frequencies of 1, 2, and 4 Hz compared to 16 and 32 Hz.
Bioassay perfusion of EDRF isolated from porcine thoracic aorta directly inhibited 2-[14C]-norepinephrine efflux in pulmonary arteries and veins.

Structured PICO

Population

Isolated canine pulmonary arteries and veins

Intervention

Intact endothelium (or exposure to EDRF from porcine thoracic aorta)

Comparator

Endothelium rubbed blood vessels (endothelium removed)

Outcome

Efflux of radiolabeled norepinephrine (2-[14C]-NE) during transmural nerve stimulation at 1, 2, 4, 8, 16 and 32 Hz for 10 minsurrogate

The endothelium, partly through EDRF, acts as an endogenous inhibitor of sympathetic neurotransmitter release in pulmonary blood vessels.

Cite This Study

Greenberg et al. (1990) studied this question.

synapsesocial.com/papers/6a6f59f7ac440176ef280a8b https://doi.org/10.1093/ajh/3.3.211

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Release of Norepinephrine From Adrenergic Nerve Endings of Blood Vessels Is Modulated by Endothelium-Derived Relaxing Factor

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Release of Norepinephrine From Adrenergic Nerve Endings of Blood Vessels Is Modulated by Endothelium-Derived Relaxing Factor

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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