What question did this study set out to answer?

To examine the release, action, and cellular interactions of endothelium-derived relaxing factor using cultured vascular cells across multiple mammalian species.

June 1, 1987HypertensionOpen Access

Endothelium-derived relaxing factor in cultured cells.

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Population

Cultured endothelial and vascular smooth muscle cells isolated from porcine, bovine, and rat thoracic aorta

Design

Preclinical

Authors

ALAlex L. LoebUniversity of Virginia RJRoger A. JohnsJohns Hopkins University PMPeter MilnerUniversity of Liverpool

Discussion

Member takes

Implication

Supports EDRF as mediator of cGMP-dependent relaxation in vitro; leaves open its identity and relevance to human hypertension.

Key Points

To examine the release, action, and cellular interactions of endothelium-derived relaxing factor using cultured vascular cells across multiple mammalian species.
Isolated endothelial and vascular smooth muscle cells from porcine, bovine, and rat thoracic aortas across multiple culture passages and time points up to 14 days.
Performed mixed-species coculture experiments and short-term (4-hour) microcarrier coculture assays to evaluate endothelium-dependent cyclic guanosine monophosphate (cGMP) accumulation.
Perfused endothelium-covered microcarriers in a column system with agonists (bradykinin, A23187, melittin, arachidonate) to measure effluent-induced smooth muscle relaxation and cGMP elevation.
Vascular smooth muscle responsiveness to sodium nitroprusside-stimulated cGMP accumulation peaked at 12 to 14 days after plating, but bovine and porcine cells lost this response after passage five, unlike rat cells.
Mixed endothelial and smooth muscle cultures accumulated cGMP in response to endothelium-dependent vasodilators, whereas cultured endothelial cells alone showed no cGMP response.
Column superfusion of endothelial beads exposed to agonists released endothelium-derived relaxing factor, stimulating rapid cGMP accumulation and muscle relaxation that were both reversed by hydroquinone.

Structured PICO

Population

Cultured endothelial and vascular smooth muscle cells isolated from porcine, bovine, and rat thoracic aorta

Intervention

Treatment with endothelium-dependent vasodilators, sodium nitroprusside, bradykinin, calcium ionophore A23187, melittin, and arachidonate in various culture and coculture setups

Outcome

cGMP accumulation and relaxation of vascular smooth musclesurrogate

Demonstrates the in vitro release of EDRF from cultured endothelial cells and its role in stimulating cGMP accumulation and relaxation in vascular smooth muscle.

Cite This Study

Loeb et al. (1987) studied this question.

synapsesocial.com/papers/6a910d4a647f40dea9c1af52 https://doi.org/10.1161/01.hyp.9.6_pt_2.iii186

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Endothelium-derived relaxing factor in cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Endothelium-derived relaxing factor in cultured cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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