May 1, 1992AJP Heart and Circulatory Physiology

Interactions of endothelins and EDRF in bovine native endothelial cells: selective effects of endothelin-3

View Full Paper

Ask AI

Bookmark

Population

Bovine aortic intact endothelium (bovine native endothelial cells) and rat fetal lung (RFL)-6 cells

Design

Preclinical

Authors

TWTimothy D. WarnerNationwide Children's Hospital HSHarald SchmidtGeneral / Preventive / Lipids FMFerid MuradCross-Cutting Cardiology

Discussion

Member takes

Implication

Hypothesis-generating for ET isoform selectivity in endothelial signaling; leaves open clinical translation to human vascular tone.

Structured PICO

Population

Bovine aortic intact endothelium (bovine native endothelial cells) and rat fetal lung (RFL)-6 cells

Intervention

Endothelin-3 (ET-3), endothelin-1 (ET-1), calcium ionophore A23187, acetylcholine (ACh), ADP, sodium nitroprusside, L-NMA, and 8-bromo-cGMP

Outcome

Release of immunoreactive ET-1 (ir-ET-1) and EDRF (measured as accumulated NO2- and NO3- or cGMP accumulation)surrogate

ET-3 selectively stimulates EDRF release in bovine endothelial cells, while EDRF/NO pathways inhibit ET-1 release, demonstrating complex feedback between these vascular mediators.

Cite This Study

Warner et al. (1992) studied this question.

synapsesocial.com/papers/6a8985b75c4c6b099dec738d https://doi.org/10.1152/ajpheart.1992.262.5.h1600

View Full Paper

Ask AI

Bookmark

Interactions of endothelins and EDRF in bovine native endothelial cells: selective effects of endothelin-3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Interactions of endothelins and EDRF in bovine native endothelial cells: selective effects of endothelin-3

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider