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January 1, 1995Nucleic Acids ResearchOpen Access

Proteolytic cleavage of initiation factor elF-4γ in the reticulocyte lysate inhibits translation of capped mRNAs but enhances that of uncapped mRNAs

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Population

in vitro translation systems (reticulocyte lysate)

Comparison

depletion of eIF-4 gamma using a transcription… vs untreated system / addition of eIF-4 complex…

Design

Preclinical

Authors

TOTheophile OhimannUniversity of SussexMRMichael RauBayer (United States)SMSimon MorleyUniversity College London Hospitals NHS Foundation Trust

Discussion

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Overview

May alter mRNA therapeutic selectivity in models; leaves open cardiac translation effects in vivo.

Structured PICO

P
Population
in vitro translation systems (reticulocyte lysate)
I
Intervention
depletion of eIF-4 gamma using a transcription plasmid encoding Leader (L) protease
C
Comparator
untreated system / addition of eIF-4 complex containing intact gamma-subunit
O
Outcome
translation of capped vs uncapped mRNAssurrogate

Proteolytic degradation of eIF-4 gamma inhibits translation of capped mRNAs but stimulates translation of uncapped mRNAs in vitro.

Cite This Study

Ohimann et al. (1995) studied this question.

synapsesocial.com/papers/6a8212646cfba4e31a9fd866https://doi.org/10.1093/nar/23.3.334
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.1982 · 590 citations
  2. 2Low efficiency of the 5' nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells1994 · 119 citations
  3. 3Dependence of the adenovirus tripartite leader on the p220 submit of eukaryotic initiation factor 4F during in vitro translation1992 · 26 citations
  4. 4A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control1990 · 183 citations
  5. 5Eukaryotic initiation factors-4E and -4F stimulate 5' cap-dependent as well as internal initiation of protein synthesis.1992 · 66 citations