July 14, 2022Experimental and Therapeutic MedicineOpen Access

Treatment with a combination of Sophora flavescens alkaloids and Panax quinquefolium saponins significantly reduced myocardial inflammation at 21 days and delayed myocardial fibrosis at 60 days in mice with experimental autoimmune myocarditis.

Q: What is the clinical evidence from this study?

Study design: Other. Population: Experimental autoimmune myocarditis (n=96). Intervention: Combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) vs. Distilled water (EAM control). Primary outcome: Myocardial inflammation and fibrosis markers (CK-MB, LDH, cTn-I, IL-1β, IL-6, TNF-α, TGF-β1, Col I, Col III) (p=<0.05).

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Why the study?

Because Panax quinquefolium saponins alleviate cardiac fibrosis, the potential effects of their combination with Sophora flavescens alkaloids (KX) on different stages of autoimmune myocarditis were investigated.

Does the combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) reduce inflammation and fibrosis in a mouse model of experimental autoimmune myocarditis?

Population

Mice with experimental autoimmune myocarditis (EAM) and controls

Comparison

KX-High (275 mg/kg) or KX-Low (138 mg/kg) vs EAM and control groups receiving distilled water

Design

Randomized animal study

Follow-up

Day 21 or 60

Key result

Treatment with a combination of Sophora flavescens alkaloids and Panax quinquefolium saponins significantly reduced myocardial inflammation at 21 days and delayed myocardial fibrosis at 60 days in mice with experimental autoimmune myocarditis.

Authors

MLMenghui Liu YLYue Lin XHXU Huibo

Discussion

Member takes

Overview

Hypothesis-generating for KX in autoimmune myocarditis models; leaves open clinical translation and human trials.

Structured PICO

Does the combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) reduce inflammation and fibrosis in a mouse model of experimental autoimmune myocarditis?

Population

96 male BALB/c mice with experimental autoimmune myocarditis, treated with KX and followed for up to 60 days.

Intervention

Combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) administered by oral gavage daily at High (275 mg/kg) or Low (138 mg/kg) doses from day 0 until sacrifice (day 21 or 60).

Comparator

EAM group receiving an equivalent volume of distilled water by oral gavage, and a healthy Control group receiving emulsion II and distilled water.

Outcome

Myocardial injury (LDH, CK-MB, cTn-I), inflammation (IL-1β, IL-6, TNF-α, NF-κB pathway), and fibrosis (TGF-β1, Col I, Col III, Smad pathway) at 21 and 60 days.surrogate

Main Result

p-value: p=<0.05

The combination of Sophora flavescens alkaloids and Panax quinquefolium saponins attenuates inflammation and fibrosis in experimental autoimmune myocarditis by modulating the NF-κB and TGF-β1 pathways.

Limitations

Lack of a positive control group.
Did not measure oxidative stress parameters.
Lack of echocardiography test results for cardiac function assessment.
Insufficient evidence to state that the individual components inhibit the first and second phases of EAM, respectively.

Cite This Study

Liu et al. (2022) studied Experimental autoimmune myocarditis (n=96). Combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) vs. Distilled water (EAM control) was evaluated on Myocardial inflammation and fibrosis markers (CK-MB, LDH, cTn-I, IL-1β, IL-6, TNF-α, TGF-β1, Col I, Col III) (p=<0.05). Treatment with a combination of Sophora flavescens alkaloids and Panax quinquefolium saponins significantly reduced myocardial inflammation at 21 days and delayed myocardial fibrosis at 60 days in mice with experimental autoimmune myocarditis.

synapsesocial.com/papers/6a82f4e624ad758e22e8db83 https://doi.org/10.3892/etm.2022.11507

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July 14, 2022Experimental and Therapeutic MedicineOpen Access

Treatment with a combination of Sophora flavescens alkaloids and Panax quinquefolium saponins significantly reduced myocardial inflammation at 21 days and delayed myocardial fibrosis at 60 days in mice with experimental autoimmune myocarditis.

View Full Paper

Ask AI

Bookmark

Why the study?

Does the combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) reduce inflammation and fibrosis in a mouse model of experimental autoimmune myocarditis?

Population

Mice with experimental autoimmune myocarditis (EAM) and controls

Comparison

KX-High (275 mg/kg) or KX-Low (138 mg/kg) vs EAM and control groups receiving distilled water

Design

Randomized animal study

Follow-up

Day 21 or 60

Key result

Authors

MLMenghui Liu YLYue Lin XHXU Huibo

Discussion

Member takes

Overview

Hypothesis-generating for KX in autoimmune myocarditis models; leaves open clinical translation and human trials.

Structured PICO

Does the combination of Sophora flavescens alkaloids and Panax quinquefolium saponins (KX) reduce inflammation and fibrosis in a mouse model of experimental autoimmune myocarditis?

Population

96 male BALB/c mice with experimental autoimmune myocarditis, treated with KX and followed for up to 60 days.

Intervention

Comparator

EAM group receiving an equivalent volume of distilled water by oral gavage, and a healthy Control group receiving emulsion II and distilled water.

Outcome

Myocardial injury (LDH, CK-MB, cTn-I), inflammation (IL-1β, IL-6, TNF-α, NF-κB pathway), and fibrosis (TGF-β1, Col I, Col III, Smad pathway) at 21 and 60 days.surrogate

Main Result

p-value: p=<0.05

Limitations

Lack of a positive control group.
Did not measure oxidative stress parameters.
Lack of echocardiography test results for cardiac function assessment.
Insufficient evidence to state that the individual components inhibit the first and second phases of EAM, respectively.

Cite This Study

synapsesocial.com/papers/6a82f4e624ad758e22e8db83 https://doi.org/10.3892/etm.2022.11507

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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