Why the study?
Facilities handling poliovirus pose a risk of unintentionally reintroducing the virus into communities after eradication and containment through unidentified contaminated materials.
Generation of CD155-deficient cell lines can prevent unintentional poliovirus propagation while allowing research on other picornaviruses.
Answers are grounded in this paper and cite their sources.
May support safer picornavirus research via CD155-deficient lines; leaves open translation to human systems or in vivo models.
After eradication and containment of wild poliovirus (PV) and cessation of oral polio vaccinations, it is critical to minimize the risk of reintroducing PV into PV-free communities via facilities that handle the virus. The potential risk of unintentional PV propagation through unidentified contaminated materials is a serious issue. This study reports the generation of HeLa and RD-A cells deficient in functional CD155 gene (∆PVR cells); these cells are not susceptible to PV but remain susceptible to other picornaviruses. These ∆PVR cells will minimize the risk of unintentional transmission of PV and support performing the experiments more safely.
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Satoshi Koike (2020) studied this question.
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