What question did this study set out to answer?

To examine the effects of cyclosporin on intracellular calcium accumulation and mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes.

June 1, 1992AJP Heart and Circulatory Physiology

Cyclosporin inhibits mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes

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Authors

RARuth A. AltschuldUniversity of Maryland, Baltimore CHCorinne M. HohlUniversity of British Columbia LCLourdes CastilloUniversidad de Valladolid

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Implication

Laboratory experiment reveals cyclosporin blocks mitochondrial calcium efflux in isolated rat cardiomyocytes, suggesting transient pore opening mediates normal calcium extrusion.

Key Points

To examine the effects of cyclosporin on intracellular calcium accumulation and mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes.
Isolated adult rat ventricular cardiomyocytes were incubated with cyclosporin or ouabain, and exchangeable intracellular Ca2+ was quantified using 45Ca2+ uptake and efflux assays.
Cells were assessed for morphological changes and pharmacologically challenged using verapamil (1 microM) or crude ruthenium red (12 microM).
Incubation with 8 microM cyclosporin more than doubled 45Ca2+ uptake in a concentration-dependent manner (apparent EC50 of 0.5 microM) while cells maintained normal rod-shaped morphology, unlike ouabain-induced round hypercontraction.
The enhanced calcium accumulation induced by cyclosporin was unaffected by 1 microM verapamil but abolished by a 5-minute preincubation with 12 microM ruthenium red.
Cyclosporin reduced the rate of 45Ca2+ efflux from prelabeled myocytes into both Ca2+-containing and Ca2+-free media, indicating inhibition of mitochondrial calcium extrusion via inner membrane pores.

Cite This Study

Altschuld et al. (1992) studied this question.

synapsesocial.com/papers/6a83be4014f94baa955bd548 https://doi.org/10.1152/ajpheart.1992.262.6.h1699

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Implication

Laboratory experiment reveals cyclosporin blocks mitochondrial calcium efflux in isolated rat cardiomyocytes, suggesting transient pore opening mediates normal calcium extrusion.

Cyclosporin inhibits mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

Cyclosporin inhibits mitochondrial calcium efflux in isolated adult rat ventricular cardiomyocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

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