Sequence analysis of cDNA's derived from the RNA of Sindbis virions and of defective interfering particles
Population
Sindbis virus virions, defective interfering particles, and two Sindbis virus variants
Comparison
Sequence analysis of RNA vs RNA of the parental wild-type Sindbis virus
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Animal models reveal complex DI RNA generation; leaves open relevance to human viral pathogenesis or interventions.
Structured PICO
P
Population
Sindbis virus virions, defective interfering (DI) particles, and two Sindbis virus variants (one derived from persistently infected BHK cells for 16 months)
I
Intervention
Sequence analysis of RNA (dideoxy chain terminating method, restriction endonuclease digestion, direct chemical sequencing)
C
Comparator
RNA of the parental wild-type Sindbis virus
O
Outcome
RNA sequence differences and structural rearrangementssurrogate
Defective interfering particles of Sindbis virus are generated through complex multiple deletion steps and sequence rearrangements rather than a single deletional event.
Cite This Study
Monroe et al. (1982) studied this question.