Why the study?
Does the developed reverse transcription multiplex PCR protocol allow for rapid detection and differentiation of polioviruses from nonpoliovirus enteroviruses in clinical and environmental samples?
Population
Clinical or environmental specimens (stool, sewage) and 36 poliovirus strains tested
Design
Preclinical
Authors
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May accelerate poliovirus surveillance in samples; leaves open clinical validation before diagnostic adoption.
Does the developed reverse transcription multiplex PCR protocol allow for rapid detection and differentiation of polioviruses from nonpoliovirus enteroviruses in clinical and environmental samples?
The developed RT-multiplex PCR protocol enables rapid (within 24 h) and robust differentiation of polioviruses from other enteroviruses in clinical and environmental samples.
Egger et al. (1995) studied this question.
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