What question did this study set out to answer?

To determine the sensitivity and selectivity of putative vesicular sodium-calcium exchange inhibitors against electrogenic membrane currents in isolated cardiac myocytes.

October 1, 1986Circulation ResearchOpen Access

Membrane electrical properties of vesicular Na-Ca exchange inhibitors in single atrial myocytes.

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Population

Single frog atrial cells (myocytes)

Design

Preclinical

Authors

DBDavid R. BielefeldLockheed Martin (United States)RHR. W. HadleyCorewell Health PVPeter VassilevBulgarian Academy of Sciences

Discussion

Member takes

Implication

Lack of selectivity questions utility of these agents as Na-Ca exchange probes; leaves open need for specific inhibitors in cardiac research.

Key Points

To determine the sensitivity and selectivity of putative vesicular sodium-calcium exchange inhibitors against electrogenic membrane currents in isolated cardiac myocytes.
Loaded isolated frog atrial myocytes with sodium via the sodium ionophore monensin to elicit electrogenic creep currents.
Tested the inhibitory effects of dodecylamine, quinacrine, and 3',4'-dichlorobenzamil on myocardial creep currents and isolated membrane calcium and potassium currents.
Dodecylamine, quinacrine, and 3',4'-dichlorobenzamil blocked myocardial creep currents with estimated Ki values of 3 µM, 10 µM, and 4 µM, respectively.
Dodecylamine and 3',4'-dichlorobenzamil concurrently inhibited myocardial calcium and potassium currents over the same concentration range that blocked exchange-mediated creep currents.

Structured PICO

Population

Single frog atrial cells (myocytes)

Intervention

Exposure to Na-Ca exchange inhibitors (dodecylamine, quinacrine, and 3',4'-dichlorobenzamil) after inducing creep currents with the Na ionophore monensin

Outcome

Inhibition of myocardial creep currents and effects on myocardial Ca and K currentssurrogate

Vesicular Na-Ca exchange inhibitors lack specificity, as they also inhibit myocardial Ca and K currents, questioning their utility as selective experimental probes.

Cite This Study

Bielefeld et al. (1986) studied this question.

synapsesocial.com/papers/6a868cb607201076d354497f https://doi.org/10.1161/01.res.59.4.381

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Implication

Lack of selectivity questions utility of these agents as Na-Ca exchange probes; leaves open need for specific inhibitors in cardiac research.

Membrane electrical properties of vesicular Na-Ca exchange inhibitors in single atrial myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Membrane electrical properties of vesicular Na-Ca exchange inhibitors in single atrial myocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider