Why the study?
How does the fluorescent-antibody technique compare to neutralization for measuring antibody levels against coxsackie B viruses types 1-5 in human sera?
How does the fluorescent-antibody technique compare to neutralization for measuring antibody levels against coxsackie B viruses types 1-5 in human sera?
The fluorescent-antibody technique is less sensitive and less specific than neutralization for detecting coxsackie B virus antibodies.
Fluorescent-antibody technique warrants caution in coxsackie B serology; leaves open need for modern validation studies.
Twenty pairs of acute and convalescent sera were tested for antibody to the coxsackie B viruses types 1-5 by neutralization and fluorescent-antibody techniques. Results by fluorescence were difficult to read and sensitivity was therefore reduced. Rises by neutralization were type specific in the majority of cases, while those by fluorescence were always against more than one viral type.
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Macwilliam et al. (1974) studied this question.
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