What question did this study set out to answer?

To evaluate the regulatory effects of angiotensin II on H+-ATPase activity and proton secretion mechanisms in isolated proximal tubule fragments.

August 4, 1998Proceedings of the National Academy of SciencesOpen Access

Angiotensin II stimulates vesicular H ⁺ -ATPase in rat proximal tubular cells

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Authors

CWCarsten A. WagnerUniversity of Zurich GGGerhard GiebischYale University FLFlorian LängUniversity of Southern California

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Implication

Experimental study reveals that angiotensin II enhances proton pump extrusion in rat kidney tubules, indicating vesicle-mediated upregulation of bicarbonate reabsorption.

Key Points

To evaluate the regulatory effects of angiotensin II on H+-ATPase activity and proton secretion mechanisms in isolated proximal tubule fragments.
Monitored intracellular pH recovery using the fluorescent probe BCECF in isolated rat proximal tubule fragments after extracellular sodium removal.
Treated tubule fragments with bafilomycin A1, chloride-free media, the chloride channel blocker NPPB, or colchicine to identify the molecular pathways mediating proton pump activation.
Angiotensin II significantly accelerated sodium-independent intracellular pH recovery in isolated proximal tubule fragments.
Stimulated proton recovery was completely abolished by bafilomycin A1, chloride removal, NPPB, and colchicine, indicating that angiotensin II recruits proton pumps through chloride-dependent vesicular insertion into the brush border membrane.

Cite This Study

Wagner et al. (1998) studied this question.

synapsesocial.com/papers/6a87fdf0be6952a959582a7d https://doi.org/10.1073/pnas.95.16.9665

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Implication

Experimental study reveals that angiotensin II enhances proton pump extrusion in rat kidney tubules, indicating vesicle-mediated upregulation of bicarbonate reabsorption.

Angiotensin II stimulates vesicular H ⁺ -ATPase in rat proximal tubular cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Angiotensin II stimulates vesicular H ⁺ -ATPase in rat proximal tubular cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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