December 1, 1998Journal of VirologyOpen Access

Interaction of Poly(rC) Binding Protein 2 with the 5′ Noncoding Region of Hepatitis A Virus RNA and Its Effects on Translation

View Full Paper

Ask AI

Bookmark

Population

FRhK-4 cells, HeLa cells, and Hepatitis A virus (HAV) RNA

Comparison

Depletion of PCBP2 from HeLa cell extracts and… vs Extracts with normal PCBP2 levels; intact HAV RNA

Design

Preclinical

Authors

Discussion

PCBP2 is required for HAV translation; leaves open whether its targeting could yield clinical antivirals.

Population

FRhK-4 cells, HeLa cells, and Hepatitis A virus (HAV) RNA

Intervention

Depletion of PCBP2 from HeLa cell extracts and addition of recombinant PCBP2; removal of 5'-terminal 138 nucleotides or entire IRES of HAV RNA

Comparator

Extracts with normal PCBP2 levels; intact HAV RNA

Outcome

HAV RNA translation activity and PCBP2 binding to the 5' noncoding regionsurrogate

PCBP2 is a required cellular factor for the translation of Hepatitis A virus RNA, binding specifically to the 5' noncoding region.

Graff et al. (1998) studied this question.

View Full Paper

Ask AI

Bookmark