What question did this study set out to answer?

To investigate the role of the sarcoplasmic reticulum in spontaneous and agonist-induced calcium transients in single smooth muscle cells from pregnant rat uterus.

March 1, 2001The Journal of PhysiologyOpen Access

Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic Ca2+ in rat uterine smooth muscle cells

View Full Paper

Ask AI

Bookmark

Population

Single smooth muscle cells from pregnant rat uterus

Design

Preclinical

Authors

ASA. ShmigolUniversity of Liverpool DEDavid EisnerElectrophysiology SWSusan WrayUniversity of Bern

Discussion

Member takes

Implication

Agonist-induced SR Ca2+ release shapes rat myometrial transients; leaves open translation to human uterine contractility.

Key Points

To investigate the role of the sarcoplasmic reticulum in spontaneous and agonist-induced calcium transients in single smooth muscle cells from pregnant rat uterus.
Simultaneously measured intraluminal sarcoplasmic reticulum ([Ca2+]L) and cytosolic ([Ca2+]i) calcium concentrations in single pregnant rat myometrial cells using dual indicators: low-affinity mag-fluo-4 and high-affinity fura-2.
Assessed calcium responses following stimulation with carbachol, ATP, and high-K+ depolarization, as well as the effects of the sarcoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin.
Carbachol and ATP exposure in the absence of extracellular Ca2+ reduced [Ca2+]L while elevating [Ca2+]i; approximately 50% refill occurred in calcium-free conditions, but full replenishment required extracellular Ca2+.
ATP induced [Ca2+]i oscillations in 4 of 15 cells accompanied by repeated luminal Ca2+ release and re-uptake, both of which were completely blocked by thapsigargin.
Spontaneous [Ca2+]i transients caused no detectable change in [Ca2+]L, whereas high-K+ depolarization elevated [Ca2+]L, and raising [Ca2+]L inhibited spontaneous activity.

Structured PICO

Population

Single smooth muscle cells from pregnant rat uterus

Intervention

Carbachol, ATP, high-K+ depolarisation, or thapsigargin

Outcome

Simultaneous changes in intraluminal sarcoplasmic reticulum ([Ca2+]L) and cytosolic ([Ca2+]i) calcium concentrationsurrogate

Demonstrates the feasibility of simultaneously measuring sarcoplasmic reticulum and cytosolic calcium in uterine smooth muscle cells to investigate their response to agonists and spontaneous activity.

Cite This Study

Shmigol et al. (2001) studied this question.

synapsesocial.com/papers/6a898e1707adfc24ebcb08e3 https://doi.org/10.1111/j.1469-7793.2001.0707h.x

View Full Paper

Ask AI

Bookmark

Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic Ca2+ in rat uterine smooth muscle cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Simultaneous measurements of changes in sarcoplasmic reticulum and cytosolic Ca2+ in rat uterine smooth muscle cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider