March 1, 1991Journal of General Virology

Construction and characterization of herpes simplex type 1 viruses without introns in immediate early gene 1

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Population

Herpes simplex virus type 1 (HSV-1) in tissue culture systems

Comparison

Recombinant HSV-1 with immediate early gene 1… vs Parent virus (wild-type HSV-1)

Design

Preclinical

Authors

RERoger D. EverettMRC University of Glasgow Centre for Virus Research

Discussion

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Implication

IE-1 introns are non-essential for HSV-1 growth in culture; leaves open roles in vivo or natural contexts.

Structured PICO

Population

Herpes simplex virus type 1 (HSV-1) in tissue culture systems

Intervention

Recombinant HSV-1 with immediate early (IE) gene 1 lacking the first, second, or both introns

Comparator

Parent virus (wild-type HSV-1)

Outcome

Viral growth, particle to p.f.u. ratio, and viral polypeptide expression (Vmw110)surrogate

Introns in HSV-1 immediate early gene 1 are not essential for viral growth or Vmw110 expression in tissue culture, though they may contribute to efficient expression in certain transfection contexts.

Cite This Study

Roger D. Everett (1991) studied this question.

synapsesocial.com/papers/6a89ce01c89a2b4713ec73dc https://doi.org/10.1099/0022-1317-72-3-651

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Construction and characterization of herpes simplex type 1 viruses without introns in immediate early gene 1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Construction and characterization of herpes simplex type 1 viruses without introns in immediate early gene 1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider