January 1, 1989Nucleic Acids ResearchOpen Access

In vitro3′ end processing and poly (A) tailing of RNA inTrypanosoma cruzi

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Population

Cell-free extracts prepared from nuclei isolated from Trypanosoma cruzi

Comparison

Incubation of synthetic RNA transcripts in the… vs Heat or pre-treatment of the extract with…

Design

Preclinical

Authors

TZTadeusz A. ZwierzynskiVirginia Commonwealth University Medical Center GWGiovanni WidmerTufts University GBGregory A. BuckVirginia Commonwealth University

Discussion

Member takes

Implication

Supports exploration of T. cruzi RNA processing as a drug target; leaves open clinical translation for Chagas disease.

Structured PICO

Population

Cell-free extracts prepared from nuclei isolated from Trypanosoma cruzi

Intervention

Incubation of synthetic RNA transcripts in the presence of ATP

Comparator

Heat or pre-treatment of the extract with Micrococcal nuclease

Outcome

3' polyadenylation activitysurrogate

Demonstrates 3' polyadenylation activity in T. cruzi nuclear extracts requiring an RNA-protein complex, elucidating mRNA processing mechanisms in the Chagas disease agent.

Cite This Study

Zwierzynski et al. (1989) studied this question.

synapsesocial.com/papers/6a8a8644eeed409805de8f9f https://doi.org/10.1093/nar/17.12.4647

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In vitro3′ end processing and poly (A) tailing of RNA inTrypanosoma cruzi

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In vitro3′ end processing and poly (A) tailing of RNA inTrypanosoma cruzi

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider