Induction of Type-C RNA Virus by Cycloheximide: Increased Expression of Virus-Specific RNA
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Population
Mouse cells including BALB/c mouse embryo cells, continuous mouse lines BALB/3T3, BALB/3T12-3, K-BALB, and…
Comparison
Cycloheximide exposure for 12-24 hours. vs Untreated cells, or cells treated with RNA…
Design
Preclinical
Authors
Discussion
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Implication
Cycloheximide may derepress type-C viruses in murine cells via new RNA synthesis; leaves open relevance to human retroviral latency or therapy.
Structured PICO
P
Population
Mouse cells including BALB/c mouse embryo cells, continuous mouse lines BALB/3T3, BALB/3T12-3, K-BALB, and normal rat kidney (NRK) line.
I
Intervention
Cycloheximide (e.g., 10 µg/ml) exposure for 12-24 hours.
C
Comparator
Untreated cells, or cells treated with RNA synthesis inhibitors (actinomycin D or cordycepin).
O
Outcome
Virus activation (fraction of virus-induced cells) and cellular concentration of virus-specific RNA.surrogate
Cycloheximide induces type-C RNA virus in mouse cells by a mechanism requiring de novo RNA synthesis during drug exposure, suggesting it prevents synthesis of a labile repressor protein.
Cite This Study
Aaronson et al. (1974) studied this question.
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