January 1, 1990Molecular and Cellular Biology

Molecular and Functional Analysis of the Muscle-Specific Promoter Region of the Duchenne Muscular Dystrophy Gene

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Population

Myogenic cell lines and a cosmid clone containing the first exon of the Duchenne muscular dystrophy (DMD) gene

Design

Preclinical

Authors

HKHenry J. KlamutOntario Institute for Cancer Research SGSuman B. GangopadhyayUniversity of Calgary RWRonald G. WortonHospital for Sick Children

Discussion

Member takes

Implication

Does not alter DMD management; extends promoter mapping for future muscle-targeted gene therapy vectors.

Structured PICO

Population

Myogenic cell lines and a cosmid clone containing the first exon of the Duchenne muscular dystrophy (DMD) gene

Intervention

Chimeric chloramphenicol acetyltransferase (CAT) gene constructs containing upstream sequences of the DMD gene

Outcome

Muscle-specific transcription of chimeric CAT gene constructs and identification of cis-acting sequencessurrogate

Identifies the minimal promoter region (149 bp) and key regulatory elements required for muscle-specific transcription of the Duchenne muscular dystrophy gene.

Cite This Study

Klamut et al. (1990) studied this question.

synapsesocial.com/papers/6a8b0ab9c12eaf65c8b1f677 https://doi.org/10.1128/mcb.10.1.193-205.1990

View Full Paper

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Molecular and Functional Analysis of the Muscle-Specific Promoter Region of the Duchenne Muscular Dystrophy Gene

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Molecular and Functional Analysis of the Muscle-Specific Promoter Region of the Duchenne Muscular Dystrophy Gene

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider