What question did this study set out to answer?

To simultaneously examine the effects of nitroglycerin on cytosolic calcium concentrations and mechanical tension development in porcine coronary arterial smooth muscle.

November 1, 1990British Journal of PharmacologyOpen Access

Front‐surface fluorometry with fura‐2 and effects of nitroglycerin on cytosolic calcium concentrations and on tension in the coronary artery of the pig

View Full Paper

Ask AI

Bookmark

Why the study?

Does nitroglycerin reduce cytosolic calcium concentrations and tension in the coronary artery of the pig?

Population

Fura-2-loaded strips of the coronary artery of the pig

Comparison

Nitroglycerin (NG) vs Resting state, high K+ depolarization, or…

Design

Preclinical

Authors

SAShimako AbeNakamura Gakuen University HKHideo KanaideKyushu University MNMotoomi NakamuraBoston University

Discussion

Member takes

Implication

Supports Ca2+-independent relaxation by nitroglycerin in porcine coronaries; leaves open translation to clinical vasomotor control.

Key Points

To simultaneously examine the effects of nitroglycerin on cytosolic calcium concentrations and mechanical tension development in porcine coronary arterial smooth muscle.
Loaded pig coronary artery strips with the fluorescent calcium indicator fura-2 and monitored cytosolic calcium ([Ca2+]i) alongside mechanical tension using front-surface fluorometry.
Evaluated responses to high potassium depolarization, histamine, and caffeine in the presence and absence of extracellular calcium with graded concentrations of nitroglycerin.
Nitroglycerin concentration-dependently reduced [Ca2+]i and tension during resting conditions and during high potassium or histamine stimulation.
The extent of tension reduction exceeded the level expected solely from the observed decrease in [Ca2+]i relative to potassium-depolarization relationships.
In calcium-free media, nitroglycerin depleted histamine-sensitive calcium stores and suppressed caffeine-induced tension without altering [Ca2+]i.

Structured PICO

Does nitroglycerin reduce cytosolic calcium concentrations and tension in the coronary artery of the pig?

Population

Fura-2-loaded strips of the coronary artery of the pig

Intervention

Nitroglycerin (NG)

Comparator

Resting state, high K+ depolarization, or histamine stimulation (baseline/control conditions)

Outcome

Cytosolic Ca2+ concentrations ([Ca2+]i) and tension developmentsurrogate

Nitroglycerin relaxes the porcine coronary artery not only by reducing cytosolic calcium but also by directly modulating contractile elements via calcium-independent second messengers.

Cite This Study

Abe et al. (1990) studied this question.

synapsesocial.com/papers/6a8be882510c7253b5ded8ff https://doi.org/10.1111/j.1476-5381.1990.tb14118.x

View Full Paper

Ask AI

Bookmark