Key Points
- Determine whether ingestion of poliomyelitis virus leads to early viremia during the incubation period prior to paralytic onset in nonhuman primates.
- Orally inoculated 10 cynomolgus monkeys and 4 chimpanzees with poliomyelitis virus strains, specifically Brunhilde type (Egypt) and Lansing type (Y-SK).
- Assayed serial blood samples collected over incubation days 1 through 7 for virus presence and tracked the interval to paralysis.
- Viremia occurred in 7 of 10 cynomolgus monkeys and 3 of 4 chimpanzees, presenting on days 4, 5, and 6 post-infection across both viral strains.
- Virus was not recovered from blood taken during days 1 to 3 post-feeding, nor on day 7 in the instances evaluated.
- The interval between detectable viremia and the appearance of paralysis ranged from 3 to 7 days in cynomolgus monkeys.
Structured PICO
PPopulation10 cynomolgus monkeys and 4 chimpanzees
IInterventionOral infection with poliomyelitis virus (Brunhilde type [Egypt] or Lansing type [Y-SK])
OOutcomePresence of viremia and time to appearance of paralysissurrogate
Demonstrates that viremia occurs early in the incubation period following oral infection with poliomyelitis virus in primate models.