Endothelin-1 stimulates both cAMP formation and phosphatidylinositol hydrolysis in embryonic bovine tracheal cells, indicating dual receptor coupling.
Hypothesis-generating for dual ET-1 receptor coupling in airway cells; requires confirmation in human models.
Embryonic bovine tracheal (EBTr) cells were found to possess receptors for endothelin (ET) of ET-1-selective (ETA) subtype with a Kd for ET-1 of 114 pM and a Bmax of 12.9 fmol/10(5) cells. Stimulation of EBTr cells with 100 pM to 100 nM ET-1 increased the contents of both inositol phosphates and cAMP in a concentration-dependent manner, indicating that the receptors are coupled to both phosphatidylinositol hydrolysis and cAMP formation in EBTr cells.
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Oda et al. (1992) studied this question.
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