Simultaneous Measurement of Membrane Capacitance and Whole Cell Currents during Cell Swelling in Macrovascular Endothelium

View Full Paper

Ask AI

Bookmark

Population

Cultured endothelial cells from bovine pulmonary artery (CPAE cells), n=22

Comparison

Cell swelling vs Isotonic solution (baseline)

Design

Preclinical

Authors

Discussion

Swelling-induced Cl- currents in endothelial cells arise independently of vesicle fusion; leaves open the precise activation mechanism.

Population

Cultured endothelial cells from bovine pulmonary artery (CPAE cells), n=22

Intervention

Cell swelling (with or without vesicular transport inhibition using brefeldin A)

Comparator

Isotonic solution (baseline)

Outcome

Membrane capacitance (Cm) and whole-cell Cl- currentssurrogate

Activation of swelling-induced Cl- currents in endothelial cells is not mediated by the incorporation of ion channels via vesicle fusion.

Heinke et al. (1997) studied this question.

View Full Paper

Ask AI

Bookmark