Key result
Temperature variation has a very small effect on the releasable elastic energy within the power stroke of a single Myosin II, which remains approximately 42 zJ across different temperatures.
Theoretical modeling suggests that while temperature affects myosin II swing rates and force, it has minimal impact on releasable elastic energy during the power stroke.
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Findings in non-mammalian models should not yet inform cardiac therapies; hypothesis-generating for temperature effects on human myosin.
Myosin IIs in the skeletal muscle are highly efficient nanoscale machines evolved in nature. Understanding how they function can not only bring insights into various biological processes but also provide guidelines to engineer synthetic nanoscale motors working in the vicinity of thermal noise. Though it was clearly demonstrated that the behavior of a skeletal muscle fiber, or that of a single myosin was strongly affected by the temperature, how exactly the temperature affects the kinetics of a single myosin is not fully understood. By adapting the newly developed transitional state model, which successfully explained the intriguing motor force regulation during skeletal muscle contraction, here we systematically explain how exactly the power stroke of a single myosin proceeds, with the consideration of the chemomechanical regulation of sub-steps within the stroke. The adapted theory is then utilized to investigate the temperature effect on various aspects of the power stroke. Our analysis suggests that, though swing rates, the isometric force, and the maximal stroke size all strongly vary with the temperature, the temperature can have a very small effect on the releasable elastic energy within the power stroke.
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Dong et al. (2016) studied this question. Temperature variation was evaluated on Releasable elastic energy within the power stroke. Temperature variation has a very small effect on the releasable elastic energy within the power stroke of a single Myosin II, which remains approximately 42 zJ across different temperatures.
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