May 19, 2006The Journal of PhysiologyOpen Access

Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single‐molecule experiments

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Population

Regulated actin filaments placed on heavy meromyosin attached to a glass surface

Design

Preclinical

Authors

MKMasataka KawaiUniversity of Iowa TKTakanori KidoWaseda University MVMartin VögelAltair Engineering (United States)

Discussion

Member takes

Implication

May refine cross-bridge models in muscle research; leaves open translation to human cardiac contractility.

Structured PICO

Population

Regulated actin filaments (reconstituted thin filaments) placed on heavy meromyosin (HMM) attached to a glass surface

Intervention

Temperature variation (20-35 degrees C) and addition of tropomyosin (Tm) with troponin (Tn) and Ca2+ or truncated mutant Delta23Tm

Outcome

Temperature dependence of force, sliding velocity, and number of cross-bridges at the single-molecule levelsurrogate

Single-molecule experiments show that the force generated by each actomyosin cross-bridge is independent of temperature, and that tropomyosin positively regulates actin to enhance force generation.

Cite This Study

Kawai et al. (2006) studied this question.

synapsesocial.com/papers/6a91b04ba9e7dc2f4660ec42 https://doi.org/10.1113/jphysiol.2006.111708

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Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single‐molecule experiments

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single‐molecule experiments

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider