Key result
Protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide block viral RNA synthesis in poliovirus-infected HeLa-S3 cells via accumulation of large precursor proteins.
Protease inhibitors block poliovirus RNA synthesis in vivo by causing accumulation of precursor proteins that inhibit viral RNA polymerase.
In poliovirus-infected HeLa-S3 cells, the protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide cause an accumulation of large precursor proteins, and they block viral RNA synthesis most probably via these products. Viral RNA polymerase activity can, however, be extracted by detergent containing buffer (Tris/Nonidet P-40, deoxycholate) from the inhibited cells. Only cytoplasmic extracts from infected cells treated with tolylsulfonyl-phenylalanyl chloromethyl ketone or iodoacetamide contain a protein which inhibits the in vitro polymerase reaction.
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Röder et al. (1974) studied Poliovirus infection. Protease inhibitors (tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide) was evaluated on Viral RNA synthesis and polymerase activity. Protease inhibitors tolylsulfonyl-phenylalanyl chloromethyl ketone and iodoacetamide block viral RNA synthesis in poliovirus-infected HeLa-S3 cells via accumulation of large precursor proteins.
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