What question did this study set out to answer?

To investigate the relationship between the angiotensin-converting enzyme (ACE) gene insertion/deletion polymorphism and the progression of diabetic nephropathy in type 2 diabetes mellitus.

March 1, 2019International Journal of Diabetes and MetabolismOpen Access

ACE Insertion/Deletion gene polymorphism and genomic sequence in Diabetic nephropathy

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Why the study?

Modulation of ACE gene activity might affect renal disease progression, but its exact role in type 2 diabetic nephropathy remains unclear.

Does ACE gene insertion/deletion polymorphism correlate with diabetic nephropathy severity and associated complications in T2DM patients?

Population

30 T2DM cases

Comparison

ACE gene polymorphism alleles (I/I vs I/D vs D/D)

Design

Prospective, cross-sectional, observational study

Authors

SHSF HaqueAligarh Muslim University AMA. MhmadAligarh Muslim University AKA.U. KhanAligarh Muslim University

Discussion

Member takes

Implication

DD genotype of ACE I/D polymorphism was associated with progressive diabetic nephropathy; leaves open its role in risk stratification pending prospective validation.

Key Points

To investigate the relationship between the angiotensin-converting enzyme (ACE) gene insertion/deletion polymorphism and the progression of diabetic nephropathy in type 2 diabetes mellitus.
Conducted a prospective cross-sectional observational study of 30 patients with type 2 diabetes mellitus (mean age 45.21 ± 2.34 years) assessing albuminuria, renal function, blood pressure, and lipid profiles.
Extracted genomic DNA and amplified intron 16 of the ACE gene using polymerase chain reaction (PCR) followed by sequencing to identify insertion (I) and deletion (D) alleles.
Genotyping identified 8 patients with the I/I allele, 18 with the I/D allele, and 4 with the D/D allele.
Micro- and macro-vascular complications were significantly more prevalent in patients with the D/D genotype compared to other genotypes (P=0.012).
Patients with the D/D genotype had significantly elevated urinary albumin excretion, systolic and diastolic blood pressure, triglycerides, serum creatinine, and LDL-C compared to the I/I and I/D groups (P<0.005), whereas 75% of I/I carriers showed delayed onset of overt albuminuria (P<0.005).

Structured PICO

Does ACE gene insertion/deletion polymorphism correlate with diabetic nephropathy severity and associated complications in T2DM patients?

Population

30 patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy, mean age 45.21±2.34 years.

Intervention

ACE gene insertion/deletion polymorphism (I/I, I/D, D/D alleles)

Comparator

Comparison among I/I, I/D, and D/D genotypes

Outcome

Micro- and macro-vascular complications, albuminuria, renal dysfunction, hypertension, and dyslipidemiasurrogate

The DD allele of the ACE gene is associated with more progressive diabetic nephropathy and higher rates of micro- and macro-vascular complications in patients with type 2 diabetes.

Cite This Study

Haque et al. (2019) studied this question.

synapsesocial.com/papers/6a911f97efab70d99b73e76d https://doi.org/10.1159/000497700

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Implication

DD genotype of ACE I/D polymorphism was associated with progressive diabetic nephropathy; leaves open its role in risk stratification pending prospective validation.

ACE Insertion/Deletion gene polymorphism and genomic sequence in Diabetic nephropathy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

ACE Insertion/Deletion gene polymorphism and genomic sequence in Diabetic nephropathy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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