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February 1, 1997Biochemical JournalOpen Access

Effect of lipid composition on lipoprotein lipase activity measured by a continuous fluorescence assay: effect of cholesterol supports an interfacial surface penetration model

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Population

Triolein-containing emulsion particles of defined composition (in vitro biochemical model)

Comparison

Modification of lipid composition and addition… vs Emulsion particles without cholesterol; standard…

Design

Preclinical

Authors

LLL. Isabel B. LOBOUniversity of SouthamptonDWDavid C. WiltonUniversity of Southampton

Discussion

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Implication

Supports interfacial LPL model in vitro; hypothesis-generating and requires in vivo confirmation before any clinical relevance.

Structured PICO

P
Population
Triolein-containing emulsion particles of defined composition (in vitro biochemical model)
I
Intervention
Modification of lipid composition (increasing cholesterol/egg-phosphatidylcholine ratio up to 1:1 molar ratio) and addition of apolipoprotein CII or Naja naja phospholipase A2
C
Comparator
Emulsion particles without cholesterol; standard radiochemical assay
O
Outcome
Lipoprotein lipase (LPL) activity (rate of hydrolysis of triolein)surrogate

Cholesterol stimulates lipoprotein lipase activity in vitro, supporting an interfacial penetration model where the enzyme's active site gains direct access to triacylglycerol in the lipoprotein core.

Cite This Study

LOBO et al. (1997) studied this question.

synapsesocial.com/papers/6a91611a87dfe5d232f133dbhttps://doi.org/10.1042/bj3210829
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Mechanism of Action of Lipoprotein Lipase on Triolein Particles: Effect of Apolipoprotein C-II11984 · 30 citations
  2. 2Functional topology of a surface loop shielding the catalytic center in lipoprotein lipase1992 · 37 citations
  3. 3Effects of cholesterol content on the metabolism of protein-free emulsion models of lipoproteins1986 · 115 citations
  4. 4Triacylglycerol and phospholipid hydrolysis in human plasma lipoproteins: role of lipoprotein and hepatic lipase1992 · 78 citations
  5. 5Fat Emulsions with Added Free Cholesterol or Fatty Acid Cholesteryl Esters1977 · 7 citations