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March 1, 1977Archives of Neurology

Simultaneous Determination of Skeletal Muscle Fiber, Types I, IIA, and IIB by Histochemistry

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Population

Human, rat, rabbit, and porcine skeletal muscle

Design

Preclinical

Key result

A simplified histochemical technique using glycine-formaldehyde-calcium preincubation clearly differentiates type I, type IIA, and IIB skeletal muscle fibers in human, rat, rabbit, and porcine muscle.

Authors

GTGary L. TunellMHM. N. Hart

Discussion

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Overview

This study describes a simplified histochemical technique for the simultaneous determination of three skeletal muscle fiber types from a single section.

Structured PICO

P
Population
Human, rat, rabbit, and porcine skeletal muscle
I
Intervention
Preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (ATPase) incubation
O
Outcome
Differentiation of type I, type IIA, and IIB fibers from a single section

This study describes a simplified histochemical technique for the simultaneous determination of three skeletal muscle fiber types from a single section.

Cite This Study

Tunell et al. (1977) studied this question. Glycine-formaldehyde-calcium preincubation was evaluated on Differentiation of type I, type IIA, and IIB fibers. A simplified histochemical technique using glycine-formaldehyde-calcium preincubation clearly differentiates type I, type IIA, and IIB skeletal muscle fibers in human, rat, rabbit, and porcine muscle.

synapsesocial.com/papers/6a932197ad4c69d0b0424416https://doi.org/10.1001/archneur.1977.00500150057011
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