Key result
A simplified histochemical technique using glycine-formaldehyde-calcium preincubation clearly differentiates type I, type IIA, and IIB skeletal muscle fibers in human, rat, rabbit, and porcine muscle.
This study describes a simplified histochemical technique for the simultaneous determination of three skeletal muscle fiber types from a single section.
Answers are grounded in this paper and cite their sources.
May support simplified fiber typing in research; leaves open clinical validation and adoption.
We describe a simplified technique for the histochemical determination of three fiber types from a single section of skeletal muscle. Preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (ATPase) incubation clearly differentiates type I, type IIA, and IIB fibers in human, rat, rabbit, and porcine muscle. In addition, glycine-formaldehyde-calcium preincubation offers better preservation of cytoarchitecture and standardization of incubation time.
No takes yet. Share an insight, caveat, or question.
Tunell et al. (1977) studied this question. Glycine-formaldehyde-calcium preincubation was evaluated on Differentiation of type I, type IIA, and IIB fibers. A simplified histochemical technique using glycine-formaldehyde-calcium preincubation clearly differentiates type I, type IIA, and IIB skeletal muscle fibers in human, rat, rabbit, and porcine muscle.
Synapse has enriched 10 related papers. Consider them for comparative context: