Key result
Two missense alleles of the Drosophila melanogaster act88F actin gene completely abolished flight ability and disrupted myofibril structure, while only weakly inducing heat shock protein synthesis.
Two specific missense mutations in the Drosophila act88F actin gene disrupt myofibril structure but do not strongly induce heat shock protein synthesis.
We have characterized two extant mutations of the flight muscle-specific act88F actin gene of Drosophila melanogaster. Both defective alleles were recovered from flightless mutants isolated previously (K. Mogami and Y. Hotta, Mol. Gen. Genet. 183:409-417, 1981). By directly sequencing the mutant alleles, we demonstrated that in act88FIfm(3)2 a single G-C to A-T transition converted arginine-28 to cysteine and that in act88FIfm(3)4 a single A-T to T-A transversion changed isoleucine-76 to phenylalanine. We showed that the actins encoded by either allele were strongly antimorphic. Mutant alleles effectively disrupted myofibril structure and function in the flight muscles of strains having the diploid complement of wild-type act88F genes. However, unlike antimorphic actins encoded by three previously characterized act88F alleles, neither that encoded by act88FIfm(3)2 nor that encoded by act88FIfm(3)4 was a strong inducer of heat shock protein synthesis.
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Karlik et al. (1987) studied Drosophila melanogaster flight muscle mutations. act88F missense alleles (Ifm(3)2 and Ifm(3)4) vs. Wild-type act88F alleles was evaluated on Normal flight ability. Two missense alleles of the Drosophila melanogaster act88F actin gene completely abolished flight ability and disrupted myofibril structure, while only weakly inducing heat shock protein synthesis.
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