Key result
Except for FPIA, assays like ULTRA-FPIA (SDC decreased from 2.00 to 0.98 ng/ml with escalating FAB) accurately measured free serum digoxin concentrations in the presence of digoxin Fab antibodies.
Why the study?
How do digoxin Fab antibodies affect the measurement of free serum digoxin concentration across five different immunoassays?
How do digoxin Fab antibodies affect the measurement of free serum digoxin concentration across five different immunoassays?
ULTRA-FPIA, STRATUS, RIA, and EMIT can measure free serum digoxin concentrations in the presence of digoxin Fab antibodies, whereas FPIA cannot.
Authors
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ULTRA-FPIA and similar assays may enable free digoxin monitoring during Fab therapy; leaves open human translation pending clinical validation.
Ujhelyi et al. (1990) studied Digoxin toxicity. Digoxin Fab antibodies (FAB) vs. No FAB was evaluated on Free serum digoxin concentration (SDC). Except for FPIA, assays like ULTRA-FPIA (SDC decreased from 2.00 to 0.98 ng/ml with escalating FAB) accurately measured free serum digoxin concentrations in the presence of digoxin Fab antibodies.