June 1, 1993Journal of Biomedical Materials Research
Detection of remnant proteolytic activities in unimplanted glutaraldehyde‐treated bovine pericardium and explanted cardiac bioprostheses
Why the study?
Does glutaraldehyde-treated bovine pericardium contain active proteolytic enzymes that might contribute to bioprosthetic valve degeneration?
Population
Soluble and insoluble preparations obtained from both unimplanted and implanted glutaraldehyde-treated…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Active proteases in fixed pericardium may contribute to bioprosthetic degeneration; leaves open whether inhibition improves clinical durability.
Structured PICO
Does glutaraldehyde-treated bovine pericardium contain active proteolytic enzymes that might contribute to bioprosthetic valve degeneration?
P
Population
Soluble and insoluble preparations obtained from both unimplanted and implanted glutaraldehyde-treated bovine parietal pericardium and explanted cardiac bioprostheses
I
Intervention
In vitro analysis of proteolytic enzyme activity using colorimetric techniques, SDS-PAGE, and gel filtration
O
Outcome
Presence and activity of proteolytic enzymes (trypsin-like proteases, cathepsin-like proteases, and collagenase)surrogate
Active proteases remain in glutaraldehyde-fixed bovine pericardium and explanted bioprostheses, potentially contributing to intrinsic extracellular matrix degeneration and valve failure.
Cite This Study
Simionescu et al. (1993) studied this question.