Key result
The RNA/DNA-hybrid detection assay specifically and sensitively detected hepatitis A virus negative-strand RNA with a lower detection limit of 100 amol, comparable to PCR methods.
Population
Hepatitis A virus (HAV) RNA
Design
Preclinical
Authors
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May advance specific HAV RNA detection tools; leaves open clinical diagnostic utility pending human validation.
The study describes a novel RNA-DNA hybridization and luminescence detection method for specifically detecting low amounts of viral single-stranded RNA species, such as HAV negative-strand RNA.
Schmitz et al. (1998) studied Hepatitis A virus (HAV) infection (in vitro). RNA/DNA-hybrid detection assay (HDA) vs. Positive-strand RNA / PCR method was evaluated on Detection of HAV negative-strand RNA. The RNA/DNA-hybrid detection assay specifically and sensitively detected hepatitis A virus negative-strand RNA with a lower detection limit of 100 amol, comparable to PCR methods.
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