Key result
In vitro translation assays demonstrated that at least 90% of internal ribosome entry on FMDV RNA occurs at the Lab AUG, with ribosomes accessing the downstream Lb site via linear scanning.
The study demonstrates that ribosomes access the FMDV Lb initiation site by linear scanning from the upstream Lab site, rather than by independent internal entry, and that initiation site selection is governed by local sequence context and downstream secondary structure.
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Challenges prevailing IRES models in FMDV; leaves open in vivo relevance for antiviral strategies.
Pöyry et al. (2011) studied Foot-and-Mouth Disease Virus (FMDV) translation initiation. Mutations and structural modifications to FMDV RNA IRES vs. Wild-type FMDV RNA was evaluated on Ratio of translation products from Lab and Lb initiation sites and combined product yield. In vitro translation assays demonstrated that at least 90% of internal ribosome entry on FMDV RNA occurs at the Lab AUG, with ribosomes accessing the downstream Lb site via linear scanning.
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