October 15, 1996Journal of Clinical InvestigationOpen Access

Autoimmunity in Chagas' disease. Identification of cardiac myosin-B13 Trypanosoma cruzi protein crossreactive T cell clones in heart lesions of a chronic Chagas' cardiomyopathy patient.

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Population

Heart-infiltrating T cell clones obtained from endomyocardial biopsy of a 35-year-old male patient with…

Comparison

In vitro exposure to human cardiac myosin heavy… vs Negative controls.

Design

Preclinical

Authors

ECEdécio Cunha‐NetoHeart Failure / Cardiomyopathy VCVerônica CoelhoInstituto Nacional de Tecnologia LGLuiza GuilhermeUniversidade de São Paulo

Discussion

Member takes

Implication

Supports molecular mimicry in Chagas cardiomyopathy; hypothesis-generating and requires human validation before clinical consideration.

Structured PICO

Population

Heart-infiltrating T cell clones obtained from endomyocardial biopsy of a 35-year-old male patient with severe chronic Chagas' disease cardiomyopathy (severe heart failure and dilated cardiomyopathy, positive serology for T. cruzi).

Intervention

In vitro exposure to human cardiac myosin heavy chain, human skeletal myosin, recombinant T. cruzi antigens (including B13, B12, CRA, FRA, JL5), and whole T. cruzi trypomastigote lysate.

Comparator

Negative controls (nonrecombinant beta-galactosidase and clean nitrocellulose strips).

Outcome

T cell proliferation measured by [3H]thymidine incorporation (counts per minute).surrogate

The identification of heart-infiltrating T cell clones that cross-react with both cardiac myosin and the T. cruzi B13 protein provides direct evidence supporting molecular mimicry in the pathogenesis of chronic Chagas' disease cardiomyopathy.

Limitations

Low absolute cpm values due to expansion and cloning protocol in the absence of in vitro selection with the test antigen
Use of antigen-bound nitrocellulose particles generating low proliferative values
Lower counting efficiency of the gas-based direct beta emission counter compared to conventional liquid scintillation

Cite This Study

Cunha‐Neto et al. (1996) studied this question.

synapsesocial.com/papers/6a95877f7ee17f80122cbfc7 https://doi.org/10.1172/jci118969

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Autoimmunity in Chagas' disease. Identification of cardiac myosin-B13 Trypanosoma cruzi protein crossreactive T cell clones in heart lesions of a chronic Chagas' cardiomyopathy patient.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Autoimmunity in Chagas' disease. Identification of cardiac myosin-B13 Trypanosoma cruzi protein crossreactive T cell clones in heart lesions of a chronic Chagas' cardiomyopathy patient.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

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