The study characterizes a specific 7 S hamster antibody that reacts with the SV40 tumor antigen.
Answers are grounded in this paper and cite their sources.
May guide antigen-specific assays in viral tumor models; extends foundational observations but should not yet inform clinical practice.
Summary Fractionation of sera obtained from hamsters bearing tumors induced by SV40-transformed cells by diethylaminoethyl (DEAE)-cellulose and Sephadex G 200 chromatography yielded an antibody with sedimentation coefficient of 7 S. This antibody reacted specifically with SV40 tumor antigen in complement-fixation and immunofluorescence tests. The antibody in whole serum and in isolated fractions also sedimented as 7 S when centrifuged in sucrose density gradients; antibody obtained from animals bearing tumors of various size had similar properties. Immunoelectrophoretic analysis of the antibody isolated on DEAE-cellulose revealed the presence of two 7 S γ-globulins; antibody activity has been associated with the slower migrating globulin. The antibody activity was resistant to inactivation by 0.1 M mercaptoethanol.
No takes yet. Share an insight, caveat, or question.
Satvir S. Tevethia (1967) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: