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March 11, 2023Problems of VirologyOpen Access

Mumps virus (Paramyxoviridae: Orthorubulavirus: Mumps orthorubulavirus) genotyping as a component of laboratory confirmation of infection

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Authors

TRT. S. RubalskaiaDED. V. ErokhovPZPolina E. Zherdeva

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Overview

Molecular surveillance study identifies distinct mumps virus genotype G lineages in infected patients, highlighting unexpected genetic diversity in localized outbreaks.

Key Points

  • To characterize the molecular genetic profiles and phylogenetic relationships of mumps virus strains circulating in the Russian Federation in 2022.
  • Collected cheek swab samples from N=11 patients presenting with clinically diagnosed mumps in the Russian Federation.
  • Extracted viral RNA directly from clinical specimens and conducted reverse transcription-polymerase chain reaction (RT-PCR).
  • Sequenced amplification products using the Sanger method and performed phylogenetic analysis using MEGA-X software.
  • Identified mumps virus genotype G in all 11 evaluated clinical samples.
  • Resolved two distinct viral phylogenetic clusters (G-1 and G-2) that exhibited significant divergence from strains circulating in other countries.

Cite This Study

Rubalskaia et al. (2023) studied this question.

synapsesocial.com/papers/6a962e1b3c640d92aed7c48ahttps://doi.org/10.36233/0507-4088-157
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Also Consider

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  1. 1Genomic diversity of mumps virus and global distribution of the 12 genotypes2014 · 129 citations
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