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Molecular techniques reveal genetic variations in the SH gene among mumps virus strains, indicating evolutionary relationships.
Background: Accurate mumps identification relies on robust genotyping. The research examined swab samples from mumps cases, focusing on cheek mucous membranes, aiming to unravel genetic makeup in the SH gene segment. Objectives: The main objectives of our research were to utilize molecular techniques for mumps identification, investigate genetic variations within the SH gene, and discern the evolutionary relationships among different mumps virus strains. Materials and Methods: RNA extracted via QIAamp viral RNA mini kit. OneStep RT-PCR amplified SH gene (7419 bp) using QIAGEN kit. SeqScape Software 3 processed genetic data, revealing mumps virus strain groupings. Iraqi strain OR413933 shared 53% similarity with LC685515.1:1-1318, categorized as G1. OR413934 exhibited a close genetic relationship with OR413933. Results: G2 cluster emerged, including OR413939, OR413935, OR413936, and OR413937 isolates, sharing lineage. The study underscores evolutionary dynamics’ exploration and biological implications, offering origin and function insights. G1 strains displayed identical sequences in the SH gene. G1 had 10 nucleotide substitutions vs. LC685507.1:11411-13635 Mumps orthorubulavirus . G2 comprised three identical strains. Research highlights genotyping’s mumps identification significance. Analysis revealed the biological relevance for comprehensive comprehension. Conclusion: The research conclude the genotyping’s role in mumps virus identification with genetic groupings and evolution’s impact.
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Abady et al. (2025) studied this question.
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