Key result
HN gene RFLP analysis successfully differentiates the Hoshino mumps vaccine strain from wild strains.
Population
Clinical samples containing mumps virus (Hoshino vaccine strain and wild strains)
Design
Other
Authors
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May enable direct Hoshino vaccine-wild strain differentiation from clinical samples; leaves open validation before routine surveillance use.
The developed RT-PCR and RFLP method allows for the direct and effective differentiation of the Hoshino mumps vaccine strain from wild strains in clinical samples.
Kashiwagi et al. (1997) studied Mumps virus infection. Restriction fragment length polymorphism (RFLP) analysis of the HN gene was evaluated on Differentiation of the Hoshino vaccine strain from wild strains. A restriction fragment length polymorphism analysis of the hemagglutinin-neuraminidase gene successfully differentiated the Hoshino mumps vaccine strain from wild strains in clinical samples.
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